Isolation of Human nm23 Genomes and Analysis of Loss of Heterozygosity in Primary Colorectal Carcinomas Using a Specific Genomic Probe1
نویسندگان
چکیده
Genomic clones of nm23-H1 and -ff2 were isolated. The nm23-H1 and -112 genes were located in a tandem array 4 kilobases apart. Each genome contained 5 exons and most of the splicing sites in the exon-intron junctions of two isotypes were essentially identicaL A probe dertved from intron 4 ofnm23-H1 was used to examine the loss ofheterozygosity (LOH) In primary colorectal carcinomas. Twenty-nine of 42 samples were in formative and LOH was found in 3 of these 29. In all three samples with LOll, cancer tissues expressed lower levels of nm23-H1 mRNA when compared with those without LOH.
منابع مشابه
Isolation of human nm23 genomes and analysis of loss of heterozygosity in primary colorectal carcinomas using a specific genomic probe.
Genomic clones of nm23-H1 and -H2 were isolated. The nm23-H1 and -H2 genes were located in a tandem array 4 kilobases apart. Each genome contained 5 exons and most of the splicing sites in the exon-intron junctions of two isotypes were essentially identical. A probe derived from intron 4 of nm23-H1 was used to examine the loss of heterozygosity (LOH) in primary colorectal carcinomas. Twenty-nin...
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